March 27, 2012

New Data Show BioCision’s CoolCell® Family of Products Outperform Traditional Cryopreservation Methods

Demonstrated 33 Percent Increase in Viable Human Embryonic Stem Cell Yields Reinforce Technology Benefit

MILL VALLEY, CALIF. – March. 27, 2011
BioCision LLC today released new data demonstrating that the company’s CoolCell® technology resulted in a 33 percent increase in human embryonic stem cell (hESC) viability when compared to common freezing methods, such as styrofoam boxes, paper towel wraps and alcohol-based methods.

Based on thermo-conductive principles and developed through precision-engineering, CoolCell is a rate-controlled cryopreservation system that ensures uniform and reproducible freezing rates for biological samples, including primary cells, cell lines, stem cells, yeast and other cell types.

“Addressing the lack of standardization in common sample handling, processing and viable long-term storage has emerged as one of the greatest challenges facing biobanks, diagnostics companies and researchers today,” said Rolf Ehrhardt, CEO, BioCision. “Scientists know that error is inherent in sample handling and can be the difference between viable and non-viable cells, particularly when handling embryonic stems cells that are extremely fragile following thawing.”[1]

Researchers set out to compare cell recovery rates post-thaw using the CoolCell method of cryopreservation and three commonly used methods with two human Embryonic Stem Cell (hESC) lines, RC-7 and RC-10. The study concluded that:

-      Results showed a 33 percent increase in viable cell count using a CoolCell (P<0.005) when compared to other methods three days post-thaw

-      The rate of stem cell adherence to tissue culture plates post-thaw was also increased when using the CoolCell compared to other methods

-      Elimination of isopropanol, and expense associated with the costs and hazards associated with use and disposal resulted in significant costs savings of $340/year per unit

- more -

The study was conducted in collaboration with scientists at Edinburgh-based Roslin Cellab, a stem cell technology company with expertise in the stem cell culture and regenerative medicine field, and sister company to Roslin Cells, a leading European and UK provider of clinical grade human embryonic stem cells.

“The results of our study show that the CoolCell technology clearly outperforms all other methods and provides excellent efficiency and reproducibility for stem cell cryopreservation and recovery,” said Aidan Courtney, Director at Roslin Cellab.

About CoolCell

CoolCell alcohol-free cell cryopreservation systems provide highly reproducible controlled-rate freezing for eukaryotic cells, including primary cells, cell lines, stem cells, PBMC, yeast and other types of cells. Used worldwide in academic, research and clinical laboratories, CoolCell is the standard for passive controlled-rate cell cryopreservation.  The CoolCell technology - using novel thermo-conductive alloy and highly insulative materials - ensures precise heat removal from each tube and standardizes the freezing experience of all cryogenic tubes in a -80˚C freezer.

Benefits include:

-      No alcohol - no ongoing expense, no maintenance, no performance variability

-      High reproducibility - identical freezing profiles every time

-      High post-thaw viability - more cells initially to get to your experiment faster

-      Variety of CoolCell modules - for 1ml to 5ml tubes, up to 30 tubes capacity

-      Several colors - for individual lab members or for easy identification

-      No pre-cooling - use off the shelf, not cold to the touch when frozen

-      No stuck lids- easy to open and re-use after five minute thaw period


About BioCision

BioCision’s mission is to standardize pre-analytical sample handling. They develop novel products that eliminate variability in common, often-overlooked, laboratory procedures. Their products are marketed globally and are widely used by the leading institutions in the biotech, pharmaceutical, clinical, diagnostic, academic and government scientific community. BioCision’s products include CoolRack®, CoolSink and ThermalTray™ laboratory tube and plate temperature standardizing modules, CoolBox ice-free benchtop coolers, CoolCell® alcohol-free cell freezing containers and TruCool™ accessories and consumables.


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[1] hESC Culture Protocols. Manual for Essential Human Embryonic Stem Cell Culture Methods Course. Accessed on 3/22/2012 at


CoolCell® Controlled-rate cell freezing equivalent to CRFs

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BioCision is a life science research and development company that develops products and solutions for process standardization throughout the healthcare industry through the application of advanced thermal regulation principles and technologies. The intuitive design and interconnectivity of BioCision products enables researchers, clinicians and manufacturers to protect the integrity of temperature-sensitive therapeutics, biological samples, and biomaterials. By comprehensively addressing temperature stability, BioCision strives to improve the success of therapeutic discovery and development and enable effective care delivery.

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